General Cleanup Technology

Biological test procedures for assessing the quality of soils and soil materials during on-site-analysis; toxic potential extractable with water (Part 2.1)

The scientific and technical goals have been achieved: the preparation of the samples, the execution of the biological tests and the evaluation of the data have been modified to such an extent that they can be conducted in laboratory containers within a short time. This considerably facilitates the evaluation of areas in which longstanding pollution is suspected.

Summary

This project formed part of the work carried out by the "ERNTE" research association. Its aim was to identify and improve the options for utilising biological test methods to evaluate contaminated soils. This sub-project particularly focused on the optimisation of the following aquatic test methods: Vibrio fischeri luminescence inhibition test according to ISO 11348 and "umu" genotoxicity tests according to ISO 13829. Both are carried out in numerous laboratories, so that round-robin tests can also be organised.
The aim was to achieve a clear reduction in the time required to prepare the samples and to carry out the tests.

Results:

  • A method for the rapid elution of soil samples has been developed.
  • It was possible to completely automate the "umu" genotoxicity test on the basis of a robot platform. Future applications will be able to make use of the pipetting stations of other service providers. This automated test can be used to evaluate chemicals and both aquatic and soil samples.
  • Our cooperation with the City of Hamburg's Urban Development and Environmental Council in the evaluation of samples from the sites in Hamburg-Grasbrook and Hamburg-Schlachthofstraße resulted in the acquisition of important experience and proof of the benefits of biological test methods. 
  • Instructions for the integration of ecotoxicological methods into the evaluation of soils are currently being drawn up on the basis of the results shown here and in cooperation with all other partners of the association.

More Project Information

Project title: Optimisation of ecotoxical test methods for routine use (ERNTE): Biological test procedures for assessing the quality of soils and soil materials during on-site-analysis; toxic potential extractable with water (Part 2.1)

Project number: 0330302

Project period: 01.04.2002 - 30.09.2005

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Source: German National Library of Science and Technology Hannover (TIB)